Join Eric Wexler for an in-depth discussion in this video Understanding gel analysis, part of Photoshop CS3 Extended: Research Methods and Workflows.
Now with Photoshop CS3's new analysis tools it might be possible to analyze gels and blots. Built into the analysis feature is the ability to collect data on the mean intensity, median intensity, maximum and minimum intensity as well as integrated density. And we'll be able to do this on the region of interest you create using Photoshop allows us to measure the amount of protein in any particular area of the band. Now this method hasn't particularly been validated, but I'm doing this to show you a possible workflow and which we'll be analyzing the background intensity to see if there's any shading artifact or any severe gradient that might impact our analysis, and then we'll go on to actually analyze the relative amount of intensity for a row of bands and be able to export the data into Excel and analyze the results.
- Conducting gel analysis
- Comparing analysis of bands
- Determining an affected area
- Outputting data
- Understanding a penetration model
- Combining images from different acquisition systems
- Recording measurements from samples
- Creating and applying a standard curve
- Determining experimental activity