Tracing a selection using the Lasso tool


show more Tracing a selection using the Lasso tool provides you with in-depth training on Photography. Taught by Eric Wexler as part of the Photoshop CS3 Extended for BioMedical Research show less
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Tracing a selection using the Lasso tool

In the previous video, we covered using Marquees to create regions of interest or areas of interest. In this video, we are going to use the Lasso tool so we are able to trace around a region of interest. So I will select the Lasso tool and we want to make sure it's the regular Lasso tool, since we are going to manually trace around this vessel. If you are working with me open HE_ NORMAL20x.TIF found in your Chapter 10 Exercise Folder. What you are seeing is a hematoxylin and eosin stained coronary vessel. We want to make sure that any measurements we perform are on the correct measurement scale. So we go to Analysis > Set Measurement Scale and select the 20x Calibration.

Now we can always use the Info panel to monitor what calibration setting we have selected. Now with this image, one area that you might want to manually trace around would be the boundary between the adventitia, here this lighter pink color, and the cardio myocytes. This would be very difficult to do in an automate...

Tracing a selection using the Lasso tool
Video duration: 3m 31s 5h 37m Intermediate

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Tracing a selection using the Lasso tool provides you with in-depth training on Photography. Taught by Eric Wexler as part of the Photoshop CS3 Extended for BioMedical Research

Subject:
Photography
Software:
Photoshop
Author:
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