Join Eric Wexler for an in-depth discussion in this video Creating a noncontiguous selection using the Magic Wand, part of Photoshop CS3 Extended for BioMedical Research.
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In the previous movie, we used the Magic Wand to measure one contiguous area.…If you are following along with me, open up Colony.tif from the Chapter 10…Exercise Files. In this movie, we are going to cover using the Magic Wand to…identify separate areas with a single click. We want to make sure that…Contiguous is checked off. Our Tolerance is on 100 will be fine for this…particular example. And it's a simple selecting the Magic Wand. And now we…click in the middle of the one of the colonies and all the other colonies have…been selected. And with this we can use the Refine Edge and we can just mask this.…
We are able to reset everything. Contract/Expand to 0, Feather to 0,…Smooth to 0, Contrast and Radius to 0.…And now by bringing the contrast up, we are going to create a much sharper edge…around each of the colonies. We can hit OK and now when we click Record…Measurements, we have a count of how many colonies are in our image, which is 15,…and the total area, in this case 233 pixels, which was covered by the colonies.…
NOTE: Actual biological research images are used for this title's examples. Some of these images, including those of internal organs and dissected animals, may be considered graphic or offensive to some viewers. Viewer discretion is strongly advised.
- Understanding imaging in biomedical research
- Getting started in Photoshop
- Organizing digital assets
- Working with image stacks
- Evaluating image color and histograms
- Modifying images for research
- Compensating for acquisition problems and limitations
- Adding reference information to images
- Sharing work
- Optimizing and creating a DICOM image or animation