Join Eric Wexler for an in-depth discussion in this video Applying a threshold to an image, part of Photoshop CS3 Extended for BioMedical Research.
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In this video, I am going to show a way to threshold an image and that's to…simplify the image and get to a point that we can binarize it, have either a…positive, which would be black in this case, or a negative which would be…white. That way we can apply image analysis to it. What we see here is the…picrosirius red-stained heart and what I want to do is to simplify the image so…that we can easily move it in and measure the red using either Photoshop now or…a different image analysis program.…
First I am going to correct the image, I will bring up the Curves command with…the Command or Ctrl, on the PC, +M and I am going to set a white point by…clicking on Set White Point eyedropper and moving it to an area which has no…tissue. Then we will hit OK and next, and I did show this previously, we are…change the way we look at this image from RGB,…and we're changing to the mode to CMYK. Then we did remember that magenta was…the most appropriate single channel to look at just the pure intensity levels…
NOTE: Actual biological research images are used for this title's examples. Some of these images, including those of internal organs and dissected animals, may be considered graphic or offensive to some viewers. Viewer discretion is strongly advised.
- Understanding imaging in biomedical research
- Getting started in Photoshop
- Organizing digital assets
- Working with image stacks
- Evaluating image color and histograms
- Modifying images for research
- Compensating for acquisition problems and limitations
- Adding reference information to images
- Sharing work
- Optimizing and creating a DICOM image or animation