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Veteran pharmaceutical research scientist and member of Adobe's Biomedical Image Advisory Group, Eric J. Wexler shares his experience creating detailed biomedical imaging in Photoshop CS3 Extended for Biomedical Research. Eric shows how to use Photoshop CS3's selection, analysis, and editing tools to evaluate an image's color composition, modify images for research, optimize exposure with levels and curves, transform images with layers, and compensate for acquisition problems and limitations. Eric also explains how to add reference information to images, annotate and optimize DICOM animations, and share finished images with colleagues. Exercise files accompany the tutorials.
NOTE: Actual biological research images are used for this title's examples. Some of these images, including those of internal organs and dissected animals, may be considered graphic or offensive to some viewers. Viewer discretion is strongly advised.
In this movie, we are going to selectively de-saturate an image,or remove the color from a region that we don't need the color in. This is good if we're going to possibly be showing some objectionable material or if your audience might be a bit squeamish. If you open XenoMouse.tif found in the Chapter 9 Exercise files, we can see the xenograft tumor in the mouse. Now we want to select that area, we will use the Elliptical Marquee tool and we will identify the region. We will adjust it, and so this is the area that we want to keep in color. The rest of the image will change to grey scale because it will tell us the location, but we don't need any color associated with it.
So what we want to do is invert the selection. Go to Select > Inverse and now the area that's selected is bounded by these marching ants and the area that is not selected is the xenograft tumor. Now, we are going to de-saturate by using an adjustment layer, and we are going to go to Black and White, and here we can see that the only remaining color of the image is the tumor. We hit OK, and now in order for us to save this as a TIF or a JPEG, we want to flatten the layers. Go to Layer, all the way down to Flatten Image and now we can save this as a JPEG by going to File > Save As, and make sure we have JPEG selected.
Now, when we hit Save, we are given an additional interface in which we can select the compression setting. In this case, even a large file is only 300 KB. So that's fine. We'll hit OK and now this is a JPEG image, and we are able to put this into a presentation and easily able to point out the xenograft tumor without seeing the blood of the intestines and other things that may be objectionable.
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