Photoshop CS3 Extended for BioMedical Research
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Using selective desaturation


From:

Photoshop CS3 Extended for BioMedical Research

with Eric Wexler

Video: Using selective desaturation

In this movie, we are going to selectively de-saturate an image,or remove the color from a region that we don't need the color in. This is good if we're going to possibly be showing some objectionable material or if your audience might be a bit squeamish. If you open XenoMouse.tif found in the Chapter 9 Exercise files, we can see the xenograft tumor in the mouse. Now we want to select that area, we will use the Elliptical Marquee tool and we will identify the region. We will adjust it, and so this is the area that we want to keep in color. The rest of the image will change to grey scale because it will tell us the location, but we don't need any color associated with it.
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  1. 7m 56s
    1. Welcome
      2m 39s
    2. Disclaimer
      1m 44s
    3. Exercise files
      45s
    4. Understanding Photoshop CS3 Extended
      1m 41s
    5. Understanding which versions are covered
      1m 7s
  2. 20m 30s
    1. Understanding imaging in biomedical research
      5m 15s
    2. Understanding research image workflows
      4m 6s
    3. Understanding image fundamentals
      3m 29s
    4. Understanding image detection
      7m 40s
  3. 16m 47s
    1. Understanding digital images
      5m 28s
    2. Understanding image file types
      6m 33s
    3. Understanding objective imaging
      4m 46s
  4. 22m 1s
    1. Understanding the default workspace
      4m 0s
    2. Creating a custom workspace
      5m 31s
    3. Working with keyboard commands
      4m 20s
    4. Customizing preferences for research
      3m 50s
    5. Understanding the History Log
      4m 20s
  5. 18m 9s
    1. Learning to always work from a copy
      2m 23s
    2. Opening files in Photoshop
      4m 13s
    3. Introduction to Adobe Bridge 2.0
      2m 25s
    4. Using the Adobe Camera Raw Converter
      3m 34s
    5. Using the DICOM Importation interface
      4m 18s
    6. Working with scanned image sets
      1m 16s
  6. 13m 46s
    1. Organizing images
      5m 0s
    2. Applying rank, keywords, and filters
      6m 9s
    3. Working with image stacks
      2m 37s
  7. 27m 54s
    1. Understanding color modes
      4m 27s
    2. Understanding the Info panel
      7m 49s
    3. Reading the Histogram panel
      5m 48s
    4. Understanding color composition with channels
      6m 9s
    5. Comparing multiple images
      3m 41s
  8. 25m 19s
    1. Resizing images
      5m 0s
    2. Resizing the image canvas
      8m 11s
    3. Joining images with compositing
      7m 8s
    4. Using Auto Align and Auto Blend
      2m 5s
    5. Applying a threshold to an image
      2m 55s
  9. 23m 45s
    1. Considering adjustments
      2m 19s
    2. Understanding exposure controls
      1m 7s
    3. Optimizing exposure with Levels
      5m 1s
    4. Optimizing exposure using Curves
      7m 24s
    5. Removing color casts
      3m 3s
    6. Reducing chromatic aberrations
      4m 51s
  10. 25m 41s
    1. Understanding layers
      4m 21s
    2. Working with adjustment layers
      1m 35s
    3. Using layers to compare histological localization
      4m 41s
    4. Optimizing a fluorescent image
      4m 27s
    5. Creating a false-color image
      4m 25s
    6. Working with Smart Objects
      4m 13s
    7. Using selective desaturation
      1m 59s
  11. 1h 0m
    1. Understanding the Analysis menu
      3m 4s
    2. Creating a calibration
      3m 56s
    3. Selecting data points
      3m 0s
    4. Using the Marquee Selection tool to isolate an area of interest
      4m 18s
    5. Tracing a selection using the Lasso tool
      3m 31s
    6. Using the Polygonal Lasso tool
      6m 47s
    7. Using the Magnetic Lasso to create an area of interest
      4m 1s
    8. Working with the Quick Select tool
      4m 11s
    9. Using the Magic Wand
      4m 11s
    10. Creating a noncontiguous selection using the Magic Wand
      1m 54s
    11. Creating a selection using Color Range
      4m 32s
    12. Using the Selection tools for visual dissection
      2m 29s
    13. Using the Count tool
      5m 59s
    14. Using the ruler tool with calibration
      4m 28s
    15. Extracting data from the Measurement Log
      3m 41s
  12. 26m 31s
    1. Adding Scale bars manually
      4m 18s
    2. Adding Scale bars automatically
      5m 20s
    3. Adding text to images
      4m 50s
    4. Adding arrows to images
      6m 29s
    5. Adding vector shapes to images
      2m 57s
    6. Adding borders to images
      2m 37s
  13. 32m 29s
    1. Creating contact sheets
      4m 8s
    2. Combining images for presentation
      9m 35s
    3. Using the Bridge Slide Show feature
      2m 58s
    4. Creating a representative grayscale image
      6m 29s
    5. Using the Print interface
      2m 47s
    6. Integrating images into Microsoft Office files
      6m 32s
  14. 15m 23s
    1. Optimizing a DICOM image
      3m 49s
    2. Creating a DICOM animation
      7m 2s
    3. Annotating and optimizing animation
      4m 32s
  15. 1m 0s
    1. Goodbye
      1m 0s

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Watch the Online Video Course Photoshop CS3 Extended for BioMedical Research
5h 37m Intermediate Jan 25, 2008

Viewers: in countries Watching now:

Veteran pharmaceutical research scientist and member of Adobe's Biomedical Image Advisory Group, Eric J. Wexler shares his experience creating detailed biomedical imaging in Photoshop CS3 Extended for Biomedical Research. Eric shows how to use Photoshop CS3's selection, analysis, and editing tools to evaluate an image's color composition, modify images for research, optimize exposure with levels and curves, transform images with layers, and compensate for acquisition problems and limitations. Eric also explains how to add reference information to images, annotate and optimize DICOM animations, and share finished images with colleagues. Exercise files accompany the tutorials.

NOTE: Actual biological research images are used for this title's examples. Some of these images, including those of internal organs and dissected animals, may be considered graphic or offensive to some viewers. Viewer discretion is strongly advised.

Topics include:
  • Understanding imaging in biomedical research
  • Getting started in Photoshop
  • Organizing digital assets
  • Working with image stacks
  • Evaluating image color and histograms
  • Modifying images for research
  • Compensating for acquisition problems and limitations
  • Adding reference information to images
  • Sharing work
  • Optimizing and creating a DICOM image or animation
Subject:
Photography
Software:
Photoshop
Author:
Eric Wexler

Using selective desaturation

In this movie, we are going to selectively de-saturate an image,or remove the color from a region that we don't need the color in. This is good if we're going to possibly be showing some objectionable material or if your audience might be a bit squeamish. If you open XenoMouse.tif found in the Chapter 9 Exercise files, we can see the xenograft tumor in the mouse. Now we want to select that area, we will use the Elliptical Marquee tool and we will identify the region. We will adjust it, and so this is the area that we want to keep in color. The rest of the image will change to grey scale because it will tell us the location, but we don't need any color associated with it.

So what we want to do is invert the selection. Go to Select > Inverse and now the area that's selected is bounded by these marching ants and the area that is not selected is the xenograft tumor. Now, we are going to de-saturate by using an adjustment layer, and we are going to go to Black and White, and here we can see that the only remaining color of the image is the tumor. We hit OK, and now in order for us to save this as a TIF or a JPEG, we want to flatten the layers. Go to Layer, all the way down to Flatten Image and now we can save this as a JPEG by going to File > Save As, and make sure we have JPEG selected.

Now, when we hit Save, we are given an additional interface in which we can select the compression setting. In this case, even a large file is only 300 KB. So that's fine. We'll hit OK and now this is a JPEG image, and we are able to put this into a presentation and easily able to point out the xenograft tumor without seeing the blood of the intestines and other things that may be objectionable.

There are currently no FAQs about Photoshop CS3 Extended for BioMedical Research.

 
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