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Veteran pharmaceutical research scientist and member of Adobe's Biomedical Image Advisory Group, Eric J. Wexler shares his experience creating detailed biomedical imaging in Photoshop CS3 Extended for Biomedical Research. Eric shows how to use Photoshop CS3's selection, analysis, and editing tools to evaluate an image's color composition, modify images for research, optimize exposure with levels and curves, transform images with layers, and compensate for acquisition problems and limitations. Eric also explains how to add reference information to images, annotate and optimize DICOM animations, and share finished images with colleagues. Exercise files accompany the tutorials.
NOTE: Actual biological research images are used for this title's examples. Some of these images, including those of internal organs and dissected animals, may be considered graphic or offensive to some viewers. Viewer discretion is strongly advised.
One other feature working with an image is that we are able to do, in a way, a visual dissection. Here we are going to identify the area of adventitia, and then invert the selection and remove all nonadventitial tissue so we can visualize the adventitia as a standalone tissue layer. If you are working along with me, open TC_Normal20x.tif, found in your Chapter 10 Exercise file folder. So we'll use our Quick Select tool. And we will increase its size by using the left square bracket. And now we are going to paint our adventitia and we are going to continue modify our Quick Select tool so it always fits within the adventitia. Now if it grabs the media like that, we want to remove that. So we can select the Subtract From Selection and we can remove the media.
And we are just going to continue to grow our selection so it encompasses all of adventitia. Again, cleaning that up and you can also use the Option or Alt key on Windows to correct. And we'll continue to add the adventitia area. And in this area we want to subtract the smooth muscle cells. So we'll remove them. And we want just make sure we have an exact match.
Again we are going to clean this up so that we don't include the media. And we are going to want to just connect these two because adventitia should go all the way around. And there, now we've identified the area of tissue which is truly adventitia. And we can go to Select > Inverse Selection. And now we are going to clear the remaining tissue. And we are left with just the adventitia.
So now you can appreciate how irregular it is, where it might go a little deeper into the cardiac tissue. But it's one way to be able to really look at a single aspect of an image. In the next video, we'll be covering the Count tool and how it can help us conduct analysis.
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