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Understanding layers

From: Photoshop CS3 Extended for BioMedical Research

Video: Understanding layers

During the previous videos, I have covered a multitude of ways that Photoshop can be used with scientific images. While running the demos, I have touched briefly on layers. Now in this chapter I will give layers its due. This is one of the most important ways Photoshop works with images. We are going to step through the Layers palette and then we will start the demoing the application of layers and methods I have used in working with images in my samples. I need to refer you to two different Lynda training videos. Deke McClelland has an entire title dedicated to the more advanced use of layers called Photoshop CS3 Channels and Masks: The Essentials, and while this is targeted at creative users, it does give a great in-depth look at the techniques based on layers and related techniques. And then possibly one of the best explanations of layers and how they work was done by the author Michael Ninness in his title on Photoshop CS2.

Understanding layers

During the previous videos, I have covered a multitude of ways that Photoshop can be used with scientific images. While running the demos, I have touched briefly on layers. Now in this chapter I will give layers its due. This is one of the most important ways Photoshop works with images. We are going to step through the Layers palette and then we will start the demoing the application of layers and methods I have used in working with images in my samples. I need to refer you to two different Lynda training videos. Deke McClelland has an entire title dedicated to the more advanced use of layers called Photoshop CS3 Channels and Masks: The Essentials, and while this is targeted at creative users, it does give a great in-depth look at the techniques based on layers and related techniques. And then possibly one of the best explanations of layers and how they work was done by the author Michael Ninness in his title on Photoshop CS2.

Now if you are working along with me, open the image PSR_Heart.psd. This is found in your Chapter 9 Exercise File folder. This is the simplest image in Photoshop, it is a picture of our subject matter and it has one layer and this is called the Background Layer, and every image whether it's a all white image, all black image, or an image with a subject has to have a Background Layer. But before we go more into details, let's separate out the Layers panel and look at the different features that we have access to. We have information about a layer and then we have the ability to control it in Blend Modes which we will be covering some uses of that, the Opacity, the ability to lock whether it's in position or making changes on the pixels, and then importantly in the bottom are Adjustment Layers, Layer Groups, the ability to create a new layer, and the ability to delete a layer. These are the important interface parts of the Layers panel.

Now we are going to look a little bit more in-depth at this background layer. It has a lock and so if we want to move it, we can't because it's locked. Background layers since they have some restrictions, the first thing I do, is I will convert this into a regular layer. I will double-click and it says New Layer and all I have to do, is hit OK, it's converted the background layer into a regular layer. Now I will able to fully modify it to my needs, whether it's moving it around or doing additional changes. Layers work like the acetate sheets we use for Overheads.

You have an image on one, it's the base and you can add additional image on top and you are able to view the different layers as light shines through and it's the same way this works in Photoshop, we can add layers and you are able to not only see what you have added but depending on the Blend Mode, see the layers underneath. The way I look at layers, it's the way that you add information to an image, whether it's side by side which we demonstrated earlier or more importantly over top of each other which I demonstrated when we created the composite image.

Here we have our base image and we are going to add another image to it. If you are working along with me, please open PSR_Hrt.TIF found in your Exercise 9 file folder. Now while we have this image open we can combine this image with the first image we have opened, and it's as simple as making sure we have the Move tool selected, Clicking on this image and dragging it to the first image. Now look automatically a new layer was created and we are able to move that layer and reposition the image without affecting the image below. So this is the simplest multi layered image. We have one picture on top of another and in this case it's totally securing what's below, but we could if we want to change the Blend Modes, to be able to see through the image using different algorithms of how that's going to be presented. And we will go over that in a later movie.

So now that we have introduced layers, we are going to see how we can use Adjustment layers to modify the way we perceive an image without actually changing the pixels of the picture.

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This video is part of

Image for Photoshop CS3 Extended for BioMedical Research
Photoshop CS3 Extended for BioMedical Research

80 video lessons · 4707 viewers

Eric Wexler
Author

 
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  1. 7m 59s
    1. Welcome
      2m 39s
    2. Disclaimer
      1m 44s
    3. Exercise files
      46s
    4. Understanding Photoshop CS3 Extended
      1m 42s
    5. Understanding which versions are covered
      1m 8s
  2. 20m 31s
    1. Understanding imaging in biomedical research
      5m 16s
    2. Understanding research image workflows
      4m 6s
    3. Understanding image fundamentals
      3m 29s
    4. Understanding image detection
      7m 40s
  3. 16m 48s
    1. Understanding digital images
      5m 28s
    2. Understanding image file types
      6m 34s
    3. Understanding objective imaging
      4m 46s
  4. 22m 2s
    1. Understanding the default workspace
      4m 0s
    2. Creating a custom workspace
      5m 31s
    3. Working with keyboard commands
      4m 20s
    4. Customizing preferences for research
      3m 51s
    5. Understanding the History Log
      4m 20s
  5. 18m 10s
    1. Learning to always work from a copy
      2m 23s
    2. Opening files in Photoshop
      4m 13s
    3. Introduction to Adobe Bridge 2.0
      2m 25s
    4. Using the Adobe Camera Raw Converter
      3m 34s
    5. Using the DICOM Importation interface
      4m 19s
    6. Working with scanned image sets
      1m 16s
  6. 13m 47s
    1. Organizing images
      5m 0s
    2. Applying rank, keywords, and filters
      6m 9s
    3. Working with image stacks
      2m 38s
  7. 27m 57s
    1. Understanding color modes
      4m 27s
    2. Understanding the Info panel
      7m 49s
    3. Reading the Histogram panel
      5m 49s
    4. Understanding color composition with channels
      6m 10s
    5. Comparing multiple images
      3m 42s
  8. 25m 17s
    1. Resizing images
      5m 1s
    2. Resizing the image canvas
      8m 11s
    3. Joining images with compositing
      7m 9s
    4. Using Auto Align and Auto Blend
      2m 1s
    5. Applying a threshold to an image
      2m 55s
  9. 23m 48s
    1. Considering adjustments
      2m 19s
    2. Understanding exposure controls
      1m 7s
    3. Optimizing exposure with Levels
      5m 1s
    4. Optimizing exposure using Curves
      7m 25s
    5. Removing color casts
      3m 4s
    6. Reducing chromatic aberrations
      4m 52s
  10. 25m 43s
    1. Understanding layers
      4m 21s
    2. Working with adjustment layers
      1m 36s
    3. Using layers to compare histological localization
      4m 42s
    4. Optimizing a fluorescent image
      4m 27s
    5. Creating a false-color image
      4m 25s
    6. Working with Smart Objects
      4m 13s
    7. Using selective desaturation
      1m 59s
  11. 1h 0m
    1. Understanding the Analysis menu
      3m 5s
    2. Creating a calibration
      3m 57s
    3. Selecting data points
      3m 0s
    4. Using the Marquee Selection tool to isolate an area of interest
      4m 19s
    5. Tracing a selection using the Lasso tool
      3m 31s
    6. Using the Polygonal Lasso tool
      6m 48s
    7. Using the Magnetic Lasso to create an area of interest
      4m 1s
    8. Working with the Quick Select tool
      4m 11s
    9. Using the Magic Wand
      4m 11s
    10. Creating a noncontiguous selection using the Magic Wand
      1m 55s
    11. Creating a selection using Color Range
      4m 33s
    12. Using the Selection tools for visual dissection
      2m 30s
    13. Using the Count tool
      5m 59s
    14. Using the ruler tool with calibration
      4m 28s
    15. Extracting data from the Measurement Log
      3m 41s
  12. 26m 32s
    1. Adding Scale bars manually
      4m 18s
    2. Adding Scale bars automatically
      5m 21s
    3. Adding text to images
      4m 50s
    4. Adding arrows to images
      6m 29s
    5. Adding vector shapes to images
      2m 57s
    6. Adding borders to images
      2m 37s
  13. 32m 32s
    1. Creating contact sheets
      4m 9s
    2. Combining images for presentation
      9m 35s
    3. Using the Bridge Slide Show feature
      2m 58s
    4. Creating a representative grayscale image
      6m 30s
    5. Using the Print interface
      2m 47s
    6. Integrating images into Microsoft Office files
      6m 33s
  14. 15m 24s
    1. Optimizing a DICOM image
      3m 50s
    2. Creating a DICOM animation
      7m 2s
    3. Annotating and optimizing animation
      4m 32s
  15. 1m 1s
    1. Goodbye
      1m 1s

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