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In Photoshop CS3 Extended Projects: Research Methods and Workflows, veteran research scientist and Adobe Biomedical Image Advisory Group member Eric J. Wexler shows how to use Photoshop CS3 for detailed research and presentations with hands-on examples. Eric evaluates gel images, penetration model samples, autoradiographs, and measurement recording and workflows, he also demonstrates how to create and apply a standard curve. Exercise files accompany the course.
Now with Photoshop CS3's new analysis tools it might be possible to analyze gels and blots. Built into the analysis feature is the ability to collect data on the mean intensity, median intensity, maximum and minimum intensity as well as integrated density. And we'll be able to do this on the region of interest you create using Photoshop allows us to measure the amount of protein in any particular area of the band. Now this method hasn't particularly been validated, but I'm doing this to show you a possible workflow and which we'll be analyzing the background intensity to see if there's any shading artifact or any severe gradient that might impact our analysis, and
then we'll go on to actually analyze the relative amount of intensity for a row of bands and be able to export the data into Excel and analyze the results.
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