Photoshop CS3 Extended for BioMedical Research
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Resizing images


From:

Photoshop CS3 Extended for BioMedical Research

with Eric Wexler

Video: Resizing images

Over the next several videos, we are going to be covering how to use Photoshop to change your image to make it better suited for a research purpose. Acquisition systems produce images that need to be modified to suit researcher's needs. Changing image size and resolution was one of the main reasons I started to use Photoshop back at version 3.0. I seemed to always be resizing images to fit a need. Whether fitting them into a report or to match images from other sources. Also, it's a useful way to make the image smaller, reducing the memory usage. This will speed up processing time. I only do this though when it's proper that I change the resolution of the image. Again, this can dramatically decrease the size of the image.
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  1. 7m 56s
    1. Welcome
      2m 39s
    2. Disclaimer
      1m 44s
    3. Exercise files
      45s
    4. Understanding Photoshop CS3 Extended
      1m 41s
    5. Understanding which versions are covered
      1m 7s
  2. 20m 30s
    1. Understanding imaging in biomedical research
      5m 15s
    2. Understanding research image workflows
      4m 6s
    3. Understanding image fundamentals
      3m 29s
    4. Understanding image detection
      7m 40s
  3. 16m 47s
    1. Understanding digital images
      5m 28s
    2. Understanding image file types
      6m 33s
    3. Understanding objective imaging
      4m 46s
  4. 22m 1s
    1. Understanding the default workspace
      4m 0s
    2. Creating a custom workspace
      5m 31s
    3. Working with keyboard commands
      4m 20s
    4. Customizing preferences for research
      3m 50s
    5. Understanding the History Log
      4m 20s
  5. 18m 9s
    1. Learning to always work from a copy
      2m 23s
    2. Opening files in Photoshop
      4m 13s
    3. Introduction to Adobe Bridge 2.0
      2m 25s
    4. Using the Adobe Camera Raw Converter
      3m 34s
    5. Using the DICOM Importation interface
      4m 18s
    6. Working with scanned image sets
      1m 16s
  6. 13m 46s
    1. Organizing images
      5m 0s
    2. Applying rank, keywords, and filters
      6m 9s
    3. Working with image stacks
      2m 37s
  7. 27m 54s
    1. Understanding color modes
      4m 27s
    2. Understanding the Info panel
      7m 49s
    3. Reading the Histogram panel
      5m 48s
    4. Understanding color composition with channels
      6m 9s
    5. Comparing multiple images
      3m 41s
  8. 25m 19s
    1. Resizing images
      5m 0s
    2. Resizing the image canvas
      8m 11s
    3. Joining images with compositing
      7m 8s
    4. Using Auto Align and Auto Blend
      2m 5s
    5. Applying a threshold to an image
      2m 55s
  9. 23m 45s
    1. Considering adjustments
      2m 19s
    2. Understanding exposure controls
      1m 7s
    3. Optimizing exposure with Levels
      5m 1s
    4. Optimizing exposure using Curves
      7m 24s
    5. Removing color casts
      3m 3s
    6. Reducing chromatic aberrations
      4m 51s
  10. 25m 41s
    1. Understanding layers
      4m 21s
    2. Working with adjustment layers
      1m 35s
    3. Using layers to compare histological localization
      4m 41s
    4. Optimizing a fluorescent image
      4m 27s
    5. Creating a false-color image
      4m 25s
    6. Working with Smart Objects
      4m 13s
    7. Using selective desaturation
      1m 59s
  11. 1h 0m
    1. Understanding the Analysis menu
      3m 4s
    2. Creating a calibration
      3m 56s
    3. Selecting data points
      3m 0s
    4. Using the Marquee Selection tool to isolate an area of interest
      4m 18s
    5. Tracing a selection using the Lasso tool
      3m 31s
    6. Using the Polygonal Lasso tool
      6m 47s
    7. Using the Magnetic Lasso to create an area of interest
      4m 1s
    8. Working with the Quick Select tool
      4m 11s
    9. Using the Magic Wand
      4m 11s
    10. Creating a noncontiguous selection using the Magic Wand
      1m 54s
    11. Creating a selection using Color Range
      4m 32s
    12. Using the Selection tools for visual dissection
      2m 29s
    13. Using the Count tool
      5m 59s
    14. Using the ruler tool with calibration
      4m 28s
    15. Extracting data from the Measurement Log
      3m 41s
  12. 26m 31s
    1. Adding Scale bars manually
      4m 18s
    2. Adding Scale bars automatically
      5m 20s
    3. Adding text to images
      4m 50s
    4. Adding arrows to images
      6m 29s
    5. Adding vector shapes to images
      2m 57s
    6. Adding borders to images
      2m 37s
  13. 32m 29s
    1. Creating contact sheets
      4m 8s
    2. Combining images for presentation
      9m 35s
    3. Using the Bridge Slide Show feature
      2m 58s
    4. Creating a representative grayscale image
      6m 29s
    5. Using the Print interface
      2m 47s
    6. Integrating images into Microsoft Office files
      6m 32s
  14. 15m 23s
    1. Optimizing a DICOM image
      3m 49s
    2. Creating a DICOM animation
      7m 2s
    3. Annotating and optimizing animation
      4m 32s
  15. 1m 0s
    1. Goodbye
      1m 0s

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Watch the Online Video Course Photoshop CS3 Extended for BioMedical Research
5h 37m Intermediate Jan 25, 2008

Viewers: in countries Watching now:

Veteran pharmaceutical research scientist and member of Adobe's Biomedical Image Advisory Group, Eric J. Wexler shares his experience creating detailed biomedical imaging in Photoshop CS3 Extended for Biomedical Research. Eric shows how to use Photoshop CS3's selection, analysis, and editing tools to evaluate an image's color composition, modify images for research, optimize exposure with levels and curves, transform images with layers, and compensate for acquisition problems and limitations. Eric also explains how to add reference information to images, annotate and optimize DICOM animations, and share finished images with colleagues. Exercise files accompany the tutorials.

NOTE: Actual biological research images are used for this title's examples. Some of these images, including those of internal organs and dissected animals, may be considered graphic or offensive to some viewers. Viewer discretion is strongly advised.

Topics include:
  • Understanding imaging in biomedical research
  • Getting started in Photoshop
  • Organizing digital assets
  • Working with image stacks
  • Evaluating image color and histograms
  • Modifying images for research
  • Compensating for acquisition problems and limitations
  • Adding reference information to images
  • Sharing work
  • Optimizing and creating a DICOM image or animation
Subject:
Photography
Software:
Photoshop
Author:
Eric Wexler

Resizing images

Over the next several videos, we are going to be covering how to use Photoshop to change your image to make it better suited for a research purpose. Acquisition systems produce images that need to be modified to suit researcher's needs. Changing image size and resolution was one of the main reasons I started to use Photoshop back at version 3.0. I seemed to always be resizing images to fit a need. Whether fitting them into a report or to match images from other sources. Also, it's a useful way to make the image smaller, reducing the memory usage. This will speed up processing time. I only do this though when it's proper that I change the resolution of the image. Again, this can dramatically decrease the size of the image.

I generally acquire images at a high resolution than is needed because it ends up being a time saver if we need to look closer at the samples as well as I am able to see the entire sample in a single field of view and be able to zoom in to look at any region at a higher resolution, to be able to see more detail. This also keeps a constant awareness of the location and relationship of areas because in biology, just like real estate, it's location, location, location.

So what if it compounds at a certain level in a tissue? If it is not actually near the cells that needs to be, it's of no use. That being said once I have acquired an image I use Photoshop to resize it, so that's an appropriate dimension for whatever use I have. So. if you are following along with me, open up 1105BR08 in the Chapter 7 Exercise Files folder. Now, we can go to Image > Image Size and here is the window that we are able to control the resolution and overall size of an image. We have it in Pixel Dimensions, how many pixels wide by how many pixels high, as well as the total size of the image, and this you can monitor as you are making changes to see how you are decreasing or increasing the size of the image, though in science we will want to decrease and not increase adding empty magnification.

Next, we have the Document Size and this allows us to see the width and the height of the image at a certain resolution. And in the bottom our other controls to allow us to apply the image size in different ways. We have the Scale Styles, which we can turn on and off, but more importantly we have Constrain Proportions so you don't introduce distortion on an image when you change width. You also want height to be changed at an equal ratio. So, you want to keep Constrain Proportion on.

And lastly you have the way the image is actually resampled, an algorithm that interpolates the way that pixels are going to be combined. In this case we are using Bicubic Sharpener, which is best for reduction. This is what we select in our Preferences to make sure it is our preferred algorithm for interpolation. There are four other algorithms and now they tell you exactly what they are good for: Nearest Neighbor to preserve hard edges, there is Bilinear, there is Bicubic, which is good for smooth gradients. For enlargement use Bicubic Smoother but again we are going to use Bicubic Sharpener.

Before the implementation of Bicubic Sharpener, it was best to be decreasing revolution in smaller steps, example divisible by 2 and gradually reduce the size. But now with Bicubic Sharper we'll able to do one change of resolution. That changes the image still giving us a faithful representation even though it's been reduced so dramatically. In this case, I make sure the Constraint Proportion is on and I am looking at printing this out to share this with a colleague. I know that my printer prints at 315 pixels per inch, so I'll change the Resolution to 315 and I know the Width and the Height in inches, so I'll change the centimeters to inches and that changes both and now I am going to reduce this to fit my page.

I want no dimension larger than 8 and this reduces the overall file size from 44.3 Megabytes to 19.1 Megabytes. I can hit OK. Now, the image is resized and ready for printing. So, that's an easy introduction to the resizing imaging. We are going to be demonstrating that more in depth as we go over other workflows. Now, we are going to look at change of the canvas size of an image, which is different than resizing an image.

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