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Over the next several videos, we are going to be covering how to use Photoshop to change your image to make it better suited for a research purpose. Acquisition systems produce images that need to be modified to suit researcher's needs. Changing image size and resolution was one of the main reasons I started to use Photoshop back at version 3.0. I seemed to always be resizing images to fit a need. Whether fitting them into a report or to match images from other sources. Also, it's a useful way to make the image smaller, reducing the memory usage. This will speed up processing time. I only do this though when it's proper that I change the resolution of the image. Again, this can dramatically decrease the size of the image.
I generally acquire images at a high resolution than is needed because it ends up being a time saver if we need to look closer at the samples as well as I am able to see the entire sample in a single field of view and be able to zoom in to look at any region at a higher resolution, to be able to see more detail. This also keeps a constant awareness of the location and relationship of areas because in biology, just like real estate, it's location, location, location.
So what if it compounds at a certain level in a tissue? If it is not actually near the cells that needs to be, it's of no use. That being said once I have acquired an image I use Photoshop to resize it, so that's an appropriate dimension for whatever use I have. So. if you are following along with me, open up 1105BR08 in the Chapter 7 Exercise Files folder. Now, we can go to Image > Image Size and here is the window that we are able to control the resolution and overall size of an image. We have it in Pixel Dimensions, how many pixels wide by how many pixels high, as well as the total size of the image, and this you can monitor as you are making changes to see how you are decreasing or increasing the size of the image, though in science we will want to decrease and not increase adding empty magnification.
Next, we have the Document Size and this allows us to see the width and the height of the image at a certain resolution. And in the bottom our other controls to allow us to apply the image size in different ways. We have the Scale Styles, which we can turn on and off, but more importantly we have Constrain Proportions so you don't introduce distortion on an image when you change width. You also want height to be changed at an equal ratio. So, you want to keep Constrain Proportion on.
And lastly you have the way the image is actually resampled, an algorithm that interpolates the way that pixels are going to be combined. In this case we are using Bicubic Sharpener, which is best for reduction. This is what we select in our Preferences to make sure it is our preferred algorithm for interpolation. There are four other algorithms and now they tell you exactly what they are good for: Nearest Neighbor to preserve hard edges, there is Bilinear, there is Bicubic, which is good for smooth gradients. For enlargement use Bicubic Smoother but again we are going to use Bicubic Sharpener.
Before the implementation of Bicubic Sharpener, it was best to be decreasing revolution in smaller steps, example divisible by 2 and gradually reduce the size. But now with Bicubic Sharper we'll able to do one change of resolution. That changes the image still giving us a faithful representation even though it's been reduced so dramatically. In this case, I make sure the Constraint Proportion is on and I am looking at printing this out to share this with a colleague. I know that my printer prints at 315 pixels per inch, so I'll change the Resolution to 315 and I know the Width and the Height in inches, so I'll change the centimeters to inches and that changes both and now I am going to reduce this to fit my page.
I want no dimension larger than 8 and this reduces the overall file size from 44.3 Megabytes to 19.1 Megabytes. I can hit OK. Now, the image is resized and ready for printing. So, that's an easy introduction to the resizing imaging. We are going to be demonstrating that more in depth as we go over other workflows. Now, we are going to look at change of the canvas size of an image, which is different than resizing an image.
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