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Veteran pharmaceutical research scientist and member of Adobe's Biomedical Image Advisory Group, Eric J. Wexler shares his experience creating detailed biomedical imaging in Photoshop CS3 Extended for Biomedical Research. Eric shows how to use Photoshop CS3's selection, analysis, and editing tools to evaluate an image's color composition, modify images for research, optimize exposure with levels and curves, transform images with layers, and compensate for acquisition problems and limitations. Eric also explains how to add reference information to images, annotate and optimize DICOM animations, and share finished images with colleagues. Exercise files accompany the tutorials.
NOTE: Actual biological research images are used for this title's examples. Some of these images, including those of internal organs and dissected animals, may be considered graphic or offensive to some viewers. Viewer discretion is strongly advised.
Over the past set of videos we have recorded our measurements in the Measurement Log. Now we are going to take a close look at the way that Photoshop collects the data and the way we can export it so it will be usable. In this case we have measured the area of these four myocytes and we can see that we can bring our measurement log up and we will increase its size. And we have our label Measurement 1, 2, 3 or 4, which matches the numbers we assign to each of the cells. The document we used to collect the data, the fact that the source of the information was a selection versus the Count tool or the Ruler tool. We have conformation of the scale we have used. The units, the scale factor and the fact that each of these area measurements is a single count.
It was not a combination or sum of multiple area measurements. We have our Area & Perimeter, Height and Width, and that's the height and the width of a bounding box. This is a rudimentary display of the information. We are able to re-size the columns, increase the scale here so we can see everything. Bring in the scale units and now we can see all the data we collected.
Now we have four different tools that allow us to either select all the measurements, de-select all measurements, export the selected measurements and delete all the measurements. Also what we are able to do if we de-select all the measurements is that we can collect either contiguous rows by clicking on the row header, contiguous columns by clicking on the column header and we can hold down our command on the Macintosh or Ctrl key on Windows and select non contiguous both columns or non contiguous rows. In this case we would like to select all the data we have, select all measurements and now we are going to export it. Now Photoshop exports this information in a delimited text file. So we can hit the Export All Measurements icon, we give it a name, Myocytes, and we will save this to the desktop.
We hit Save and now when we look on our desktop we have a .txt or Text file that we can open up using Excel or other spreadsheet or data analysis programs. In this case we are going to open up Excel. We go to File > Open, desktop and here is the myocytes.text file. It is All Readable Documents is enabled. We select it, we hit open and in this case, it's Delimited, we hit next and we can Tab Delimited and we can check out the preview and to make through the columns are going to go to the spreadsheet correctly, hit Next. We hit Finish and now we can put in the data we created using just Adobe Photoshop CS3 Extended right into a spreadsheet that we can now analyze the numbers, create graphs and re-arrange it to fit our research.
Now that we have made measurements and exported data, it is time to go over adding additional information to images: text, vector shapes and scale bars.
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