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In Photoshop CS3 Extended Projects: Research Methods and Workflows, veteran research scientist and Adobe Biomedical Image Advisory Group member Eric J. Wexler shows how to use Photoshop CS3 for detailed research and presentations with hands-on examples. Eric evaluates gel images, penetration model samples, autoradiographs, and measurement recording and workflows, he also demonstrates how to create and apply a standard curve. Exercise files accompany the course.
In this movie we're going to determine the amount of activity in any area of our unknown. This is our inverted cropped area.TIF tests found in the Chapter 6 exercise file. We've zoomed in and it's next to all the standards, and we can select an area by using the Rectangular Marquee and then record its measurement. In this case the median gray value is 2854.
We can go back to the Excel spreadsheet where we created an equation and that solved for X. And we can type in the median gray value that we just measured, which was 2854, we hit Return and the average activity in that area is 4.19, or 4.2 nanocuries per microgram.
So that's how you can now apply the standard curve you created using Photoshop and actually determine the amount of radioactivity or if you had the standards for it, you can do this for the amount of protein or anything that you can create a standard curve for, an intensity, you can easily determine your equation and a correlation coefficient and then apply it to any area in the image.
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