Viewers: in countries Watching now:
Veteran pharmaceutical research scientist and member of Adobe's Biomedical Image Advisory Group, Eric J. Wexler shares his experience creating detailed biomedical imaging in Photoshop CS3 Extended for Biomedical Research. Eric shows how to use Photoshop CS3's selection, analysis, and editing tools to evaluate an image's color composition, modify images for research, optimize exposure with levels and curves, transform images with layers, and compensate for acquisition problems and limitations. Eric also explains how to add reference information to images, annotate and optimize DICOM animations, and share finished images with colleagues. Exercise files accompany the tutorials.
NOTE: Actual biological research images are used for this title's examples. Some of these images, including those of internal organs and dissected animals, may be considered graphic or offensive to some viewers. Viewer discretion is strongly advised.
In this movie, we are going to use the Color Range command to collect information about the colonies. If you are following along with me, open up Colony Count in the Chapter 10 Exercise folder. Now we see groupings of colonies and we are going to use the Color Range tool to create the selection just of the colonies. So we go up to Select, pull down Color Range, and this opens up a different way to create a selection. We can change the Preview to None so we are able to actually see the picture that we are going to want to identify a certain type of color for Photoshop to trace around. In this case, we are going to be looking at the white colonies. And we have Fuzziness, which is basically how large a range around what area we identify will the selection be created. So we will drop this down to about 50, which is a good place to start. And now we bring our cursor over, click in the middle of the colony, and we can see in our Preview box that we have selected the multiple colonies. And we can also change the Selection Preview to look at it under Grayscale, or a Black or White Matte if that would optimize our viewing, or a Quick Mask. And this way we can see that we really have identified and selected the white colonies.
So with them being selected, we can hit OK. And now we see how a selection border has been created within the colonies. This is where we can use the Refine Edge to grow the selection so we can encompass more of the actual colony area. We will need to click on a double selection tool so we have access to the Refine Edge. I clicked on Magic Wand in this case. I can click on Refine Edge. And now what I will do is I will expand. I can change the View mode and I can also increase the Feather. In this case, we can see the edges do have some feathering to them. And then now we have a proper selection for most of the colonies. We can expand this just a little bit more. Now we have adjusted the Refine Edge and we see that we are still missing a little bit of the area. We are going to close this out but keep these settings, hit OK, and then we will use the actual Magic Wand tool to add additional area.
We are going to decrease the Tolerance to about 20, make sure that Contiguous, in this case, is checked On because we don't want to expand all the selection borders just for three of these colonies. So I hit Contiguous. I am going to add to the selections by using the Quick Selection tool. I will move it over. I will decrease the size of the brush by using the left square bracket. Hold down my Shift key, okay, then click to add that colony completely and totally. And I will do that again with the colony here on the right border; hold down my Shift and add. And then I will have to adjust my cursor a little further, decreasing its size by using the left square bracket and I hold the Shift key down, click, hold the Shift key down, click.
Now I am very happy with the selection of all the colonies. And I can hit Record Measurements. And this will give me a total number of Count of the colony clumps. At the same time, we will see the total Area of each of the individual colony groupings. If you want to keep a record of this, we can add a Stroke to the selection. And that's the proper way to keep a record of where our selection border lies. Go to Edit > Stroke, we keep our Width of 2 pixels. In this case, the Color would be white so we want to change the color to something that has a high contrast, to that's red and white. We could select on the green area here but if we wanted a pure green, we can decrease the red to 0, decrease the blue to 0. And now we have a pure green. If we increase this to 255, we can hit OK. And then the Blending Mode, we will keep as Normal. And the Opacity, we can drop to 50% so we are able to see the border underneath the line we are going to draw. And I am going to leave the Location as Center so the selection line will be kept in the middle of the line we draw, instead of stroking the line on the inside of the selection border or outside of the selection border. Now we hit OK. And we can deselect by Command or Ctrl+D on the PC. And now we have created a border around the region of interest that we have measured.
There are currently no FAQs about Photoshop CS3 Extended for BioMedical Research.
Access exercise files from a button right under the course name.
Search within course videos and transcripts, and jump right to the results.
Remove icons showing you already watched videos if you want to start over.
Make the video wide, narrow, full-screen, or pop the player out of the page into its own window.
Click on text in the transcript to jump to that spot in the video. As the video plays, the relevant spot in the transcript will be highlighted.