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Creating a selection using Color Range

From: Photoshop CS3 Extended for BioMedical Research

Video: Creating a selection using Color Range

In this movie, we are going to use the Color Range command to collect information about the colonies. If you are following along with me, open up Colony Count in the Chapter 10 Exercise folder. Now we see groupings of colonies and we are going to use the Color Range tool to create the selection just of the colonies. So we go up to Select, pull down Color Range, and this opens up a different way to create a selection. We can change the Preview to None so we are able to actually see the picture that we are going to want to identify a certain type of color for Photoshop to trace around. In this case, we are going to be looking at the white colonies. And we have Fuzziness, which is basically how large a range around what area we identify will the selection be created. So we will drop this down to about 50, which is a good place to start. And now we bring our cursor over, click in the middle of the colony, and we can see in our Preview box that we have selected the multiple colonies. And we can also change the Selection Preview to look at it under Grayscale, or a Black or White Matte if that would optimize our viewing, or a Quick Mask. And this way we can see that we really have identified and selected the white colonies.

Creating a selection using Color Range

In this movie, we are going to use the Color Range command to collect information about the colonies. If you are following along with me, open up Colony Count in the Chapter 10 Exercise folder. Now we see groupings of colonies and we are going to use the Color Range tool to create the selection just of the colonies. So we go up to Select, pull down Color Range, and this opens up a different way to create a selection. We can change the Preview to None so we are able to actually see the picture that we are going to want to identify a certain type of color for Photoshop to trace around. In this case, we are going to be looking at the white colonies. And we have Fuzziness, which is basically how large a range around what area we identify will the selection be created. So we will drop this down to about 50, which is a good place to start. And now we bring our cursor over, click in the middle of the colony, and we can see in our Preview box that we have selected the multiple colonies. And we can also change the Selection Preview to look at it under Grayscale, or a Black or White Matte if that would optimize our viewing, or a Quick Mask. And this way we can see that we really have identified and selected the white colonies.

So with them being selected, we can hit OK. And now we see how a selection border has been created within the colonies. This is where we can use the Refine Edge to grow the selection so we can encompass more of the actual colony area. We will need to click on a double selection tool so we have access to the Refine Edge. I clicked on Magic Wand in this case. I can click on Refine Edge. And now what I will do is I will expand. I can change the View mode and I can also increase the Feather. In this case, we can see the edges do have some feathering to them. And then now we have a proper selection for most of the colonies. We can expand this just a little bit more. Now we have adjusted the Refine Edge and we see that we are still missing a little bit of the area. We are going to close this out but keep these settings, hit OK, and then we will use the actual Magic Wand tool to add additional area.

We are going to decrease the Tolerance to about 20, make sure that Contiguous, in this case, is checked On because we don't want to expand all the selection borders just for three of these colonies. So I hit Contiguous. I am going to add to the selections by using the Quick Selection tool. I will move it over. I will decrease the size of the brush by using the left square bracket. Hold down my Shift key, okay, then click to add that colony completely and totally. And I will do that again with the colony here on the right border; hold down my Shift and add. And then I will have to adjust my cursor a little further, decreasing its size by using the left square bracket and I hold the Shift key down, click, hold the Shift key down, click.

Now I am very happy with the selection of all the colonies. And I can hit Record Measurements. And this will give me a total number of Count of the colony clumps. At the same time, we will see the total Area of each of the individual colony groupings. If you want to keep a record of this, we can add a Stroke to the selection. And that's the proper way to keep a record of where our selection border lies. Go to Edit > Stroke, we keep our Width of 2 pixels. In this case, the Color would be white so we want to change the color to something that has a high contrast, to that's red and white. We could select on the green area here but if we wanted a pure green, we can decrease the red to 0, decrease the blue to 0. And now we have a pure green. If we increase this to 255, we can hit OK. And then the Blending Mode, we will keep as Normal. And the Opacity, we can drop to 50% so we are able to see the border underneath the line we are going to draw. And I am going to leave the Location as Center so the selection line will be kept in the middle of the line we draw, instead of stroking the line on the inside of the selection border or outside of the selection border. Now we hit OK. And we can deselect by Command or Ctrl+D on the PC. And now we have created a border around the region of interest that we have measured.

Show transcript

This video is part of

Image for Photoshop CS3 Extended for BioMedical Research
Photoshop CS3 Extended for BioMedical Research

80 video lessons · 4709 viewers

Eric Wexler
Author

 
Expand all | Collapse all
  1. 7m 59s
    1. Welcome
      2m 39s
    2. Disclaimer
      1m 44s
    3. Exercise files
      46s
    4. Understanding Photoshop CS3 Extended
      1m 42s
    5. Understanding which versions are covered
      1m 8s
  2. 20m 31s
    1. Understanding imaging in biomedical research
      5m 16s
    2. Understanding research image workflows
      4m 6s
    3. Understanding image fundamentals
      3m 29s
    4. Understanding image detection
      7m 40s
  3. 16m 48s
    1. Understanding digital images
      5m 28s
    2. Understanding image file types
      6m 34s
    3. Understanding objective imaging
      4m 46s
  4. 22m 2s
    1. Understanding the default workspace
      4m 0s
    2. Creating a custom workspace
      5m 31s
    3. Working with keyboard commands
      4m 20s
    4. Customizing preferences for research
      3m 51s
    5. Understanding the History Log
      4m 20s
  5. 18m 10s
    1. Learning to always work from a copy
      2m 23s
    2. Opening files in Photoshop
      4m 13s
    3. Introduction to Adobe Bridge 2.0
      2m 25s
    4. Using the Adobe Camera Raw Converter
      3m 34s
    5. Using the DICOM Importation interface
      4m 19s
    6. Working with scanned image sets
      1m 16s
  6. 13m 47s
    1. Organizing images
      5m 0s
    2. Applying rank, keywords, and filters
      6m 9s
    3. Working with image stacks
      2m 38s
  7. 27m 57s
    1. Understanding color modes
      4m 27s
    2. Understanding the Info panel
      7m 49s
    3. Reading the Histogram panel
      5m 49s
    4. Understanding color composition with channels
      6m 10s
    5. Comparing multiple images
      3m 42s
  8. 25m 17s
    1. Resizing images
      5m 1s
    2. Resizing the image canvas
      8m 11s
    3. Joining images with compositing
      7m 9s
    4. Using Auto Align and Auto Blend
      2m 1s
    5. Applying a threshold to an image
      2m 55s
  9. 23m 48s
    1. Considering adjustments
      2m 19s
    2. Understanding exposure controls
      1m 7s
    3. Optimizing exposure with Levels
      5m 1s
    4. Optimizing exposure using Curves
      7m 25s
    5. Removing color casts
      3m 4s
    6. Reducing chromatic aberrations
      4m 52s
  10. 25m 43s
    1. Understanding layers
      4m 21s
    2. Working with adjustment layers
      1m 36s
    3. Using layers to compare histological localization
      4m 42s
    4. Optimizing a fluorescent image
      4m 27s
    5. Creating a false-color image
      4m 25s
    6. Working with Smart Objects
      4m 13s
    7. Using selective desaturation
      1m 59s
  11. 1h 0m
    1. Understanding the Analysis menu
      3m 5s
    2. Creating a calibration
      3m 57s
    3. Selecting data points
      3m 0s
    4. Using the Marquee Selection tool to isolate an area of interest
      4m 19s
    5. Tracing a selection using the Lasso tool
      3m 31s
    6. Using the Polygonal Lasso tool
      6m 48s
    7. Using the Magnetic Lasso to create an area of interest
      4m 1s
    8. Working with the Quick Select tool
      4m 11s
    9. Using the Magic Wand
      4m 11s
    10. Creating a noncontiguous selection using the Magic Wand
      1m 55s
    11. Creating a selection using Color Range
      4m 33s
    12. Using the Selection tools for visual dissection
      2m 30s
    13. Using the Count tool
      5m 59s
    14. Using the ruler tool with calibration
      4m 28s
    15. Extracting data from the Measurement Log
      3m 41s
  12. 26m 32s
    1. Adding Scale bars manually
      4m 18s
    2. Adding Scale bars automatically
      5m 21s
    3. Adding text to images
      4m 50s
    4. Adding arrows to images
      6m 29s
    5. Adding vector shapes to images
      2m 57s
    6. Adding borders to images
      2m 37s
  13. 32m 32s
    1. Creating contact sheets
      4m 9s
    2. Combining images for presentation
      9m 35s
    3. Using the Bridge Slide Show feature
      2m 58s
    4. Creating a representative grayscale image
      6m 30s
    5. Using the Print interface
      2m 47s
    6. Integrating images into Microsoft Office files
      6m 33s
  14. 15m 24s
    1. Optimizing a DICOM image
      3m 50s
    2. Creating a DICOM animation
      7m 2s
    3. Annotating and optimizing animation
      4m 32s
  15. 1m 1s
    1. Goodbye
      1m 1s

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