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Veteran pharmaceutical research scientist and member of Adobe's Biomedical Image Advisory Group, Eric J. Wexler shares his experience creating detailed biomedical imaging in Photoshop CS3 Extended for Biomedical Research. Eric shows how to use Photoshop CS3's selection, analysis, and editing tools to evaluate an image's color composition, modify images for research, optimize exposure with levels and curves, transform images with layers, and compensate for acquisition problems and limitations. Eric also explains how to add reference information to images, annotate and optimize DICOM animations, and share finished images with colleagues. Exercise files accompany the tutorials.
NOTE: Actual biological research images are used for this title's examples. Some of these images, including those of internal organs and dissected animals, may be considered graphic or offensive to some viewers. Viewer discretion is strongly advised.
In the previous movie, we used the Magic Wand to measure one contiguous area. If you are following along with me, open up Colony.tif from the Chapter 10 Exercise Files. In this movie, we are going to cover using the Magic Wand to identify separate areas with a single click. We want to make sure that Contiguous is checked off. Our Tolerance is on 100 will be fine for this particular example. And it's a simple selecting the Magic Wand. And now we click in the middle of the one of the colonies and all the other colonies have been selected. And with this we can use the Refine Edge and we can just mask this.
We are able to reset everything. Contract/Expand to 0, Feather to 0, Smooth to 0, Contrast and Radius to 0. And now by bringing the contrast up, we are going to create a much sharper edge around each of the colonies. We can hit OK and now when we click Record Measurements, we have a count of how many colonies are in our image, which is 15, and the total area, in this case 233 pixels, which was covered by the colonies.
So this is a way to use Magic Wand to be able to quickly and easily select multiple colonies and do a count as well as collect additional information with a single tool, the Magic Wand. Now we are going to go over one last tool that we can use to create selections. In fact, it's not a tool but instead a dialog interface and it's called Color Range.
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