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Veteran pharmaceutical research scientist and member of Adobe's Biomedical Image Advisory Group, Eric J. Wexler shares his experience creating detailed biomedical imaging in Photoshop CS3 Extended for Biomedical Research. Eric shows how to use Photoshop CS3's selection, analysis, and editing tools to evaluate an image's color composition, modify images for research, optimize exposure with levels and curves, transform images with layers, and compensate for acquisition problems and limitations. Eric also explains how to add reference information to images, annotate and optimize DICOM animations, and share finished images with colleagues. Exercise files accompany the tutorials.
NOTE: Actual biological research images are used for this title's examples. Some of these images, including those of internal organs and dissected animals, may be considered graphic or offensive to some viewers. Viewer discretion is strongly advised.
In this studio we are going to create some custom workspaces. Now it is always good practice to start from the default workspace. So we are going to make sure that what we see is truly the default workspace by going to Window > Workspace and moving over and highlighting Default Workspace. Now we know it is been reset to a standard workspace. So we can make modifications but always go back to the simple starting point. Now there is another thing about workspaces that is important to realize. Let's go to Window > Workspace, and if you look below we can see some additional options including just being able to reset either the Palette Locations, Keyboard Shortcuts, or Menus. If we go further down, we can see that Adobe has actually provided some custom workspaces for us to be able to either return to the way Photoshop used to be or what I think is much more important, is to select What's New in CS3, and then we will hit Yes because this will modify.
But we can easily get back to the default workspace. So I am going to hit Return for Yes. And now, well, it does not look like anything has changed, as soon as we start pulling down the menu items, for example Analysis, which is brand new in Photoshop CS3 Extended, all the new items are highlighted in blue. Since this is a brand new menu item, everything under it is highlighted. We can also go to Layer. And we can see that there is a new Smart Filter, as well as other things that might have been updated or changed for CS3 Extended and CS3, are highlighted blue.
Another workspace setting that might be important for researches. If we go to Window > Workspace, we can see that there are different options that include Image Analysis. When we turn that one on, again we say Yes. And now, we can see that a lot of extraneous panels that are non-important for image analysis have been removed. And we also can see the brand new Measurement Log panel, here at the bottom. Now that we can see the Image Analysis workspace, this is much closer to what we might like to customize and it will save us a lot of work. At the same time, we can always go back under the Window and select this so again, it is a starting point that is etched in stone, and we can always get to and modify from there. Let us start by increasing the Measurement log and moving it a little bit. Because as we collect numbers, we want to be able to see as much of the data as possible.
Then there are some things we can turn off. Because we still do not use Paths, so I will select that, and then hit the cross here, and then the Paths panel closes. Another thing is that I will separate Channels and bring it up to be part of the Info palette. This way I can easily look at the different components of any color image. The last modification I will make is to close up this entire panel set because generally Image Analysis with Photoshop is done in a manual fashion. As well as the History Log. If I do end up needing it, I can easily pull it out from the Window menu item.
Now that the workspace is customized to the way that is most appropriate for the workflow, I want to be able to save this. And then not only use it on this computer but be able to share that with other workstations that have Photoshop installed. So I will move up to the Window, pull that down, and underneath the Workspace there is the Save Workspace command. Here, we can give it a name as well as make sure we recover the Palette Locations, if we have changed any Keyboard Shortcuts, which I will show you how to do in the next video, and any menus modification.
Now I do not want to write over the existing Image Analysis workspace. So I do want to rename this. In this case, we are going to rename it Image Analysis-, your initials; in my case, EW. Then I will hit Save. Now with any new custom workspace you can find it listed under the Window > Workspace, and it's appended to the bottom of the list. Here it is, Image Analysis- EW. If you want to save this with your colleagues or place it on another workstations, you will need to know where this preset file actually lives.
The preset file is located under the name of the user in the Library. And then you have to find Preferences, and select Adobe Photoshop CS3 Settings, and since it's a Workspace, you will be selecting this folder, and within it the actual preset is saved. Now if you already have this on your computer, you can search for it and find it. But if you want to place it into another Macintosh, you have to follow this file path and place it correctly into the Workspaces folder.
In Vista, you can find this file in the same type of path. Under the user file name, in this case, Eric Wexler, and then AppData, Roaming, followed by the Adobe folder, and then you will open up Adobe Photoshop CS3. And in that is the folder Adobe Photoshop CS3 Settings, which contains the Workspaces folder, which will include the Image Analysis- EW. And with that you can copy it and place it in the same location on any other Vista workstation that has access to a same custom workspace that you have created on a different machine.
Now that we have created and we are able to share this custom workspace, we can see how easy it is to go back to our default workspace. We are going to use the default workspace throughout the rest of the title as we demonstrate what Photoshop can do with scientific imaging.
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