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Adding Scale bars automatically

From: Photoshop CS3 Extended for BioMedical Research

Video: Adding Scale bars automatically

Under the new Analysis menu in Photoshop CS3 Extended is the new Place Scale Marker option. If the image is calibrated, this is a fast method to add a simple line scale marker to an image. If you are following along with me, open 40x_25u.tif and Myo40x.tif both found in your Chapter 11 Exercise files. The first thing we are going to do is custom calibration from our 40x_25 that we then can use to apply to the Myo40 and have Photoshop add the scale measurement marker. We go to Analysis > Set Measurement Scale > Custom.

Adding Scale bars automatically

Under the new Analysis menu in Photoshop CS3 Extended is the new Place Scale Marker option. If the image is calibrated, this is a fast method to add a simple line scale marker to an image. If you are following along with me, open 40x_25u.tif and Myo40x.tif both found in your Chapter 11 Exercise files. The first thing we are going to do is custom calibration from our 40x_25 that we then can use to apply to the Myo40 and have Photoshop add the scale measurement marker. We go to Analysis > Set Measurement Scale > Custom.

We use the Ruler tool that it automatically gives us to draw across four of the grids, holding down our Shift key so it's horizontal, and we make sure we match the edge of the line to the right hand side of the grid line so it matches our beginning point. We see our Pixel length is 405, our Logical Length, we want to make sure it's 100 and then Logical Units. We want to change this so that it's Mu, and on the Macintosh, you hold down the Option key and hit M. Alternatively, you can hold down the Alt key and type 1254 on the number pad.

Now, that we have our micron symbol, the Mu. We can type a lowercase m and we are going to save this preset. Hit Save Preset and here we are going to type in 40x_100 then the Mu symbol, the lowercase m, and we hit OK. We can hit OK again, and go to Analysis and now, under our Set Measurement Scale, we have created an additional preset. Now, we can go to the Myo40x and select that. We can assign the correct scale by going to Set Measurement Scale and selecting the 40x_100 microns. We are going to let Photoshop place a scale marker for us. Go to Analysis > Place Scale Marker. In this case, it would give us a line of exactly 100 microns. If we wanted to put in 200-micron line, we would put into, for 500-micron line, we would add 5. So it just multiples the length by the actual scale that you have created. We can either have text automatically placed. We can position it either below or above the scale mark, and we can change the color to either black or white.

In this case, we will keep it black. We hit OK and Photoshop quickly runs through an action placing in the bottom left corner, the scale bar and the text that goes with it. We will select the Move tool by hitting V. Now, we can re-position this wherever it's needed in the image. Within the layer group is a layer for the marker graphic and a layer for the text, and we are able to modify each of these by selecting the specific layer.

And in this case, we have selected the marker graphic and we can double-click the layer thumbnail. We bring up the ability to change layer styles. In this case, we are going to use it to change the color of our graphic. We go to Color Overlay, select that, we double -click and now, we can select whatever color by hitting the color box. I would like to have this graphic yellow, because that's a very high contrast and it will show up very nicely on a screen. I add 255 of green to 255 of red to get to my pure yellow, I hit OK, and I hit OK again, and I have a yellow graphic line.

I also can change the text. I will click on the text box, and the Options bar gives me the ability to customize the text. In this case, 100 microns is too small to be legible. So I can increase its size, go to the Font size. I will approximately double it, and then I can select the Move tool and position the text to the middle and lastly, I will double-click on the text layer again and I will make sure I convert it to yellow simply by adding 255 of red and 255 of green. I hit OK. In that way, I have matched the colors and I have a legible text with my scale marker.

If I needed to, I could rotate the scale marker graphic by clicking on that layer, going to Edit > Transform, I can rotate it 90 degrees, and then I can move it along the side if that's what's needed. So you are able to use the automated feature of Photoshop, but then customize it as needed by working with the different layers.

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This video is part of

Image for Photoshop CS3 Extended for BioMedical Research
Photoshop CS3 Extended for BioMedical Research

80 video lessons · 4709 viewers

Eric Wexler
Author

 
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  1. 7m 59s
    1. Welcome
      2m 39s
    2. Disclaimer
      1m 44s
    3. Exercise files
      46s
    4. Understanding Photoshop CS3 Extended
      1m 42s
    5. Understanding which versions are covered
      1m 8s
  2. 20m 31s
    1. Understanding imaging in biomedical research
      5m 16s
    2. Understanding research image workflows
      4m 6s
    3. Understanding image fundamentals
      3m 29s
    4. Understanding image detection
      7m 40s
  3. 16m 48s
    1. Understanding digital images
      5m 28s
    2. Understanding image file types
      6m 34s
    3. Understanding objective imaging
      4m 46s
  4. 22m 2s
    1. Understanding the default workspace
      4m 0s
    2. Creating a custom workspace
      5m 31s
    3. Working with keyboard commands
      4m 20s
    4. Customizing preferences for research
      3m 51s
    5. Understanding the History Log
      4m 20s
  5. 18m 10s
    1. Learning to always work from a copy
      2m 23s
    2. Opening files in Photoshop
      4m 13s
    3. Introduction to Adobe Bridge 2.0
      2m 25s
    4. Using the Adobe Camera Raw Converter
      3m 34s
    5. Using the DICOM Importation interface
      4m 19s
    6. Working with scanned image sets
      1m 16s
  6. 13m 47s
    1. Organizing images
      5m 0s
    2. Applying rank, keywords, and filters
      6m 9s
    3. Working with image stacks
      2m 38s
  7. 27m 57s
    1. Understanding color modes
      4m 27s
    2. Understanding the Info panel
      7m 49s
    3. Reading the Histogram panel
      5m 49s
    4. Understanding color composition with channels
      6m 10s
    5. Comparing multiple images
      3m 42s
  8. 25m 17s
    1. Resizing images
      5m 1s
    2. Resizing the image canvas
      8m 11s
    3. Joining images with compositing
      7m 9s
    4. Using Auto Align and Auto Blend
      2m 1s
    5. Applying a threshold to an image
      2m 55s
  9. 23m 48s
    1. Considering adjustments
      2m 19s
    2. Understanding exposure controls
      1m 7s
    3. Optimizing exposure with Levels
      5m 1s
    4. Optimizing exposure using Curves
      7m 25s
    5. Removing color casts
      3m 4s
    6. Reducing chromatic aberrations
      4m 52s
  10. 25m 43s
    1. Understanding layers
      4m 21s
    2. Working with adjustment layers
      1m 36s
    3. Using layers to compare histological localization
      4m 42s
    4. Optimizing a fluorescent image
      4m 27s
    5. Creating a false-color image
      4m 25s
    6. Working with Smart Objects
      4m 13s
    7. Using selective desaturation
      1m 59s
  11. 1h 0m
    1. Understanding the Analysis menu
      3m 5s
    2. Creating a calibration
      3m 57s
    3. Selecting data points
      3m 0s
    4. Using the Marquee Selection tool to isolate an area of interest
      4m 19s
    5. Tracing a selection using the Lasso tool
      3m 31s
    6. Using the Polygonal Lasso tool
      6m 48s
    7. Using the Magnetic Lasso to create an area of interest
      4m 1s
    8. Working with the Quick Select tool
      4m 11s
    9. Using the Magic Wand
      4m 11s
    10. Creating a noncontiguous selection using the Magic Wand
      1m 55s
    11. Creating a selection using Color Range
      4m 33s
    12. Using the Selection tools for visual dissection
      2m 30s
    13. Using the Count tool
      5m 59s
    14. Using the ruler tool with calibration
      4m 28s
    15. Extracting data from the Measurement Log
      3m 41s
  12. 26m 32s
    1. Adding Scale bars manually
      4m 18s
    2. Adding Scale bars automatically
      5m 21s
    3. Adding text to images
      4m 50s
    4. Adding arrows to images
      6m 29s
    5. Adding vector shapes to images
      2m 57s
    6. Adding borders to images
      2m 37s
  13. 32m 32s
    1. Creating contact sheets
      4m 9s
    2. Combining images for presentation
      9m 35s
    3. Using the Bridge Slide Show feature
      2m 58s
    4. Creating a representative grayscale image
      6m 30s
    5. Using the Print interface
      2m 47s
    6. Integrating images into Microsoft Office files
      6m 33s
  14. 15m 24s
    1. Optimizing a DICOM image
      3m 50s
    2. Creating a DICOM animation
      7m 2s
    3. Annotating and optimizing animation
      4m 32s
  15. 1m 1s
    1. Goodbye
      1m 1s

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