Photoshop CS3 Extended for BioMedical Research
Illustration by

Photoshop CS3 Extended for BioMedical Research

with Eric Wexler

Video: Adding Scale bars automatically

Under the new Analysis menu in Photoshop CS3 Extended is the new Place Scale Marker option. If the image is calibrated, this is a fast method to add a simple line scale marker to an image. If you are following along with me, open 40x_25u.tif and Myo40x.tif both found in your Chapter 11 Exercise files. The first thing we are going to do is custom calibration from our 40x_25 that we then can use to apply to the Myo40 and have Photoshop add the scale measurement marker. We go to Analysis > Set Measurement Scale > Custom.
Expand all | Collapse all
  1. 7m 56s
    1. Welcome
      2m 39s
    2. Disclaimer
      1m 44s
    3. Exercise files
      45s
    4. Understanding Photoshop CS3 Extended
      1m 41s
    5. Understanding which versions are covered
      1m 7s
  2. 20m 30s
    1. Understanding imaging in biomedical research
      5m 15s
    2. Understanding research image workflows
      4m 6s
    3. Understanding image fundamentals
      3m 29s
    4. Understanding image detection
      7m 40s
  3. 16m 47s
    1. Understanding digital images
      5m 28s
    2. Understanding image file types
      6m 33s
    3. Understanding objective imaging
      4m 46s
  4. 22m 1s
    1. Understanding the default workspace
      4m 0s
    2. Creating a custom workspace
      5m 31s
    3. Working with keyboard commands
      4m 20s
    4. Customizing preferences for research
      3m 50s
    5. Understanding the History Log
      4m 20s
  5. 18m 9s
    1. Learning to always work from a copy
      2m 23s
    2. Opening files in Photoshop
      4m 13s
    3. Introduction to Adobe Bridge 2.0
      2m 25s
    4. Using the Adobe Camera Raw Converter
      3m 34s
    5. Using the DICOM Importation interface
      4m 18s
    6. Working with scanned image sets
      1m 16s
  6. 13m 46s
    1. Organizing images
      5m 0s
    2. Applying rank, keywords, and filters
      6m 9s
    3. Working with image stacks
      2m 37s
  7. 27m 54s
    1. Understanding color modes
      4m 27s
    2. Understanding the Info panel
      7m 49s
    3. Reading the Histogram panel
      5m 48s
    4. Understanding color composition with channels
      6m 9s
    5. Comparing multiple images
      3m 41s
  8. 25m 19s
    1. Resizing images
      5m 0s
    2. Resizing the image canvas
      8m 11s
    3. Joining images with compositing
      7m 8s
    4. Using Auto Align and Auto Blend
      2m 5s
    5. Applying a threshold to an image
      2m 55s
  9. 23m 45s
    1. Considering adjustments
      2m 19s
    2. Understanding exposure controls
      1m 7s
    3. Optimizing exposure with Levels
      5m 1s
    4. Optimizing exposure using Curves
      7m 24s
    5. Removing color casts
      3m 3s
    6. Reducing chromatic aberrations
      4m 51s
  10. 25m 41s
    1. Understanding layers
      4m 21s
    2. Working with adjustment layers
      1m 35s
    3. Using layers to compare histological localization
      4m 41s
    4. Optimizing a fluorescent image
      4m 27s
    5. Creating a false-color image
      4m 25s
    6. Working with Smart Objects
      4m 13s
    7. Using selective desaturation
      1m 59s
  11. 1h 0m
    1. Understanding the Analysis menu
      3m 4s
    2. Creating a calibration
      3m 56s
    3. Selecting data points
      3m 0s
    4. Using the Marquee Selection tool to isolate an area of interest
      4m 18s
    5. Tracing a selection using the Lasso tool
      3m 31s
    6. Using the Polygonal Lasso tool
      6m 47s
    7. Using the Magnetic Lasso to create an area of interest
      4m 1s
    8. Working with the Quick Select tool
      4m 11s
    9. Using the Magic Wand
      4m 11s
    10. Creating a noncontiguous selection using the Magic Wand
      1m 54s
    11. Creating a selection using Color Range
      4m 32s
    12. Using the Selection tools for visual dissection
      2m 29s
    13. Using the Count tool
      5m 59s
    14. Using the ruler tool with calibration
      4m 28s
    15. Extracting data from the Measurement Log
      3m 41s
  12. 26m 31s
    1. Adding Scale bars manually
      4m 18s
    2. Adding Scale bars automatically
      5m 20s
    3. Adding text to images
      4m 50s
    4. Adding arrows to images
      6m 29s
    5. Adding vector shapes to images
      2m 57s
    6. Adding borders to images
      2m 37s
  13. 32m 29s
    1. Creating contact sheets
      4m 8s
    2. Combining images for presentation
      9m 35s
    3. Using the Bridge Slide Show feature
      2m 58s
    4. Creating a representative grayscale image
      6m 29s
    5. Using the Print interface
      2m 47s
    6. Integrating images into Microsoft Office files
      6m 32s
  14. 15m 23s
    1. Optimizing a DICOM image
      3m 49s
    2. Creating a DICOM animation
      7m 2s
    3. Annotating and optimizing animation
      4m 32s
  15. 1m 0s
    1. Goodbye
      1m 0s

Start your free trial now, and begin learning software, business and creative skills—anytime, anywhere—with video instruction from recognized industry experts.

Start Your Free Trial Now
please wait ...
Watch the Online Video Course Photoshop CS3 Extended for BioMedical Research
5h 37m Intermediate Jan 25, 2008

Viewers: in countries Watching now:

Veteran pharmaceutical research scientist and member of Adobe's Biomedical Image Advisory Group, Eric J. Wexler shares his experience creating detailed biomedical imaging in Photoshop CS3 Extended for Biomedical Research. Eric shows how to use Photoshop CS3's selection, analysis, and editing tools to evaluate an image's color composition, modify images for research, optimize exposure with levels and curves, transform images with layers, and compensate for acquisition problems and limitations. Eric also explains how to add reference information to images, annotate and optimize DICOM animations, and share finished images with colleagues. Exercise files accompany the tutorials.

NOTE: Actual biological research images are used for this title's examples. Some of these images, including those of internal organs and dissected animals, may be considered graphic or offensive to some viewers. Viewer discretion is strongly advised.

Topics include:
  • Understanding imaging in biomedical research
  • Getting started in Photoshop
  • Organizing digital assets
  • Working with image stacks
  • Evaluating image color and histograms
  • Modifying images for research
  • Compensating for acquisition problems and limitations
  • Adding reference information to images
  • Sharing work
  • Optimizing and creating a DICOM image or animation
Subject:
Photography
Software:
Photoshop
Author:
Eric Wexler

Adding Scale bars automatically

Under the new Analysis menu in Photoshop CS3 Extended is the new Place Scale Marker option. If the image is calibrated, this is a fast method to add a simple line scale marker to an image. If you are following along with me, open 40x_25u.tif and Myo40x.tif both found in your Chapter 11 Exercise files. The first thing we are going to do is custom calibration from our 40x_25 that we then can use to apply to the Myo40 and have Photoshop add the scale measurement marker. We go to Analysis > Set Measurement Scale > Custom.

We use the Ruler tool that it automatically gives us to draw across four of the grids, holding down our Shift key so it's horizontal, and we make sure we match the edge of the line to the right hand side of the grid line so it matches our beginning point. We see our Pixel length is 405, our Logical Length, we want to make sure it's 100 and then Logical Units. We want to change this so that it's Mu, and on the Macintosh, you hold down the Option key and hit M. Alternatively, you can hold down the Alt key and type 1254 on the number pad.

Now, that we have our micron symbol, the Mu. We can type a lowercase m and we are going to save this preset. Hit Save Preset and here we are going to type in 40x_100 then the Mu symbol, the lowercase m, and we hit OK. We can hit OK again, and go to Analysis and now, under our Set Measurement Scale, we have created an additional preset. Now, we can go to the Myo40x and select that. We can assign the correct scale by going to Set Measurement Scale and selecting the 40x_100 microns. We are going to let Photoshop place a scale marker for us. Go to Analysis > Place Scale Marker. In this case, it would give us a line of exactly 100 microns. If we wanted to put in 200-micron line, we would put into, for 500-micron line, we would add 5. So it just multiples the length by the actual scale that you have created. We can either have text automatically placed. We can position it either below or above the scale mark, and we can change the color to either black or white.

In this case, we will keep it black. We hit OK and Photoshop quickly runs through an action placing in the bottom left corner, the scale bar and the text that goes with it. We will select the Move tool by hitting V. Now, we can re-position this wherever it's needed in the image. Within the layer group is a layer for the marker graphic and a layer for the text, and we are able to modify each of these by selecting the specific layer.

And in this case, we have selected the marker graphic and we can double-click the layer thumbnail. We bring up the ability to change layer styles. In this case, we are going to use it to change the color of our graphic. We go to Color Overlay, select that, we double -click and now, we can select whatever color by hitting the color box. I would like to have this graphic yellow, because that's a very high contrast and it will show up very nicely on a screen. I add 255 of green to 255 of red to get to my pure yellow, I hit OK, and I hit OK again, and I have a yellow graphic line.

I also can change the text. I will click on the text box, and the Options bar gives me the ability to customize the text. In this case, 100 microns is too small to be legible. So I can increase its size, go to the Font size. I will approximately double it, and then I can select the Move tool and position the text to the middle and lastly, I will double-click on the text layer again and I will make sure I convert it to yellow simply by adding 255 of red and 255 of green. I hit OK. In that way, I have matched the colors and I have a legible text with my scale marker.

If I needed to, I could rotate the scale marker graphic by clicking on that layer, going to Edit > Transform, I can rotate it 90 degrees, and then I can move it along the side if that's what's needed. So you are able to use the automated feature of Photoshop, but then customize it as needed by working with the different layers.

There are currently no FAQs about Photoshop CS3 Extended for BioMedical Research.

 
Share a link to this course

What are exercise files?

Exercise files are the same files the author uses in the course. Save time by downloading the author's files instead of setting up your own files, and learn by following along with the instructor.

Can I take this course without the exercise files?

Yes! If you decide you would like the exercise files later, you can upgrade to a premium account any time.

Become a member Download sample files See plans and pricing

Please wait... please wait ...
Upgrade to get access to exercise files.

Exercise files video

How to use exercise files.

Learn by watching, listening, and doing, Exercise files are the same files the author uses in the course, so you can download them and follow along Premium memberships include access to all exercise files in the library.


Exercise files

Exercise files video

How to use exercise files.

For additional information on downloading and using exercise files, watch our instructional video or read the instructions in the FAQ .

This course includes free exercise files, so you can practice while you watch the course. To access all the exercise files in our library, become a Premium Member.

Join now Already a member? Log in

* Estimated file size

Are you sure you want to mark all the videos in this course as unwatched?

This will not affect your course history, your reports, or your certificates of completion for this course.


Mark all as unwatched Cancel

Congratulations

You have completed Photoshop CS3 Extended for BioMedical Research.

Return to your organization's learning portal to continue training, or close this page.


OK
Become a member to add this course to a playlist

Join today and get unlimited access to the entire library of video courses—and create as many playlists as you like.

Get started

Already a member ?

Exercise files

Learn by watching, listening, and doing! Exercise files are the same files the author uses in the course, so you can download them and follow along. Exercise files are available with all Premium memberships. Learn more

Get started

Already a Premium member?

Exercise files video

How to use exercise files.

Ask a question

Thanks for contacting us.
You’ll hear from our Customer Service team within 24 hours.

Please enter the text shown below:

The classic layout automatically defaults to the latest Flash Player.

To choose a different player, hold the cursor over your name at the top right of any lynda.com page and choose Site preferences from the dropdown menu.

Continue to classic layout Stay on new layout
Exercise files

Access exercise files from a button right under the course name.

Mark videos as unwatched

Remove icons showing you already watched videos if you want to start over.

Control your viewing experience

Make the video wide, narrow, full-screen, or pop the player out of the page into its own window.

Interactive transcripts

Click on text in the transcript to jump to that spot in the video. As the video plays, the relevant spot in the transcript will be highlighted.

Learn more, save more. Upgrade today!

Get our Annual Premium Membership at our best savings yet.

Upgrade to our Annual Premium Membership today and get even more value from your lynda.com subscription:

“In a way, I feel like you are rooting for me. Like you are really invested in my experience, and want me to get as much out of these courses as possible this is the best place to start on your journey to learning new material.”— Nadine H.

Thanks for signing up.

We’ll send you a confirmation email shortly.


Sign up and receive emails about lynda.com and our online training library:

Here’s our privacy policy with more details about how we handle your information.

Keep up with news, tips, and latest courses with emails from lynda.com.

Sign up and receive emails about lynda.com and our online training library:

Here’s our privacy policy with more details about how we handle your information.

   
submit Lightbox submit clicked
Terms and conditions of use

We've updated our terms and conditions (now called terms of service).Go
Review and accept our updated terms of service.